DBT & CSIR SPONSORED National Workshop on Plant-Biotechnology - RAPD-PCR techniques On August, 18th – 20th, 2011at ANNAMALAI UNIVERSITY.
HOST DEPARTMENT
The Department of Botany has an Outstanding Record ofpromoting Excellent Research, right from its inception. It has been recognized as DST-FIST & UGC-SAP Sponsored Department. The Department offers 2 year M.Sc. Botany and 5 year integrated M.Sc. Herbal Science Programme. In addition, M. Phil. and Ph.D. programs are offered. The Department has produced 161 Ph.Ds in a variety of botanical disciplines such as Biodiversity and its Conservation, Genetics, Cell and Molecular Biology, Microorganisms and Plant Pathology, Pollution and Environmental impacts on Ecosystem, Plant Physiology and Biotechnology, Plant Taxonomy and Plant Genomics. The Department has organized workshops, seminars and refresher courses through which a huge number of participants from various universities and institutes took part and have placed benefits.
WORKSHOP BACKGROUND
The Polymerase Chain Reaction (PCR) is a technique that permits geometric and enzymatic amplification of specific sequences of DNA. The PCR is now widely used in research laboratories and doctor's offices, relies on the ability of DNA-coping enzymes to remain stable at high temperatures. No problem for Thermus aquaticus, the sultry bacterium from yelllowstone that now helps scientist to produce million of copies of a single DNA segment in matter of hours. In nature, most organisms copy their DNA in the same way. The PCR mimics this process, only in test tubes. When any cell divides, enzymes called polymerase made a copy of the entire DNA in each chromosome. Besides, based on the applications, PCR has been modified in a number of ways to suit specific application situation, several molecular markers (RFLP, AFLP, ISSR, SSR, SNP and RAPD) also available for analyzing genetic variations at molecular level. Among these, RAPD is an inexpensive and a rapid method not requiring much information regarding the genome and has been widely used to document the genetic diversity in plants and animals. Marker-based genome analyses have become increasingly important for various fields of plant science research. In this background, the present workshop will help to provide researchers a hands-on training in molecular marker techniques and to highlight the importance of marker assisted techniques in the plant biotechnology research.
PROGRAMME SCHEDULE
Morning Session : 9.00 am to 1.00 pm;
Lunch : 1.00 pm to 2.00 pm
Afternoon Session : 2.00 pm to 6.30 pm
Tea break in the middle of each session (15min.)
WORKSHOP PROGRAMME
Besides hands-on training, participants will be encouraged to carry out the techniques themselves. The workshop will be conducted for 30 participants only. This workshop of RAPD-PCR technique will cover guest lectures and hands-on experience on various aspects of biotechnology as follows
Laboratory Safety
Tools- Knowledge-information regarding chemicals and other basic instruments required
Major instruments – PCR-thermal cycler, Electrophoresis, Gel documentation.
Chemicals preparation for
DNA isolation and purification.
DNA quantification
Agarose-gel Electrophoresis
PCR reactions
Techniques to be handled
DNA extraction: Isolation and purification of genomic DNA (plant sample).
DNA quantification in spectrophotometer : DNA qualitative analysis in agarose-gel electrophoresis
Amplification in PCR thermal cycler Initial denaturation, Denaturation, Annealing and Extension.
Agarose-gel Electrophoresis, Gel-documentation, Data scoring,Data analysis in software
Results and interpretation
REGISTRATION
The participants are requested to fill-in the Registration form and mail it to the Organizing Secretary along with a demand draft for Rs. 2000/- from Indian Bank / ICICI Bank / SBI in favour of "The Registrar, Annamalai University " payable at Annamalainagar or Chidambaram. Only 30 participants can be accommodated. The first registering persons would be preferred.
VENUE:
Department of Botany, Annamalai University, Annamalainagar-608 002, Tamilnadu.
ACCOMMODATION:
The external participants can be given accommodation for 3days in the university guest house/hostel on request.
For correspondence
Dr. R. SELVARAJ
Organizing Secretary
Professor
Department of Botany
Annamalai University
Annamalainagar – 608 002
Tamilnadu
Tel: Off : (04144)-238282 Ext.354 Fax 238080
Res: (04144)-239014
Email: selvarajphd14@yahoo.co.in
Mobile: 94429 09910
Patron : Dr. M. Ramanathan,Vice-Chancellor, Annamalai University
Vice Patron : Dr. M. Rathinasabapathi Registrar, Annamalai University
President : Dr. A.N. Kannappan Dean, Faculty of Science,
Annamalai University
Chairman : Dr. R. Panneerselvam Prof. & Head, Dept. of Botany,
Organizing Secretary : Dr. R. Selvaraj,Professor, Dept. of Botany,Annamalai University
Joint Secretary : Dr. AL. A. Chidambaram Associate Professor Dept. of Botany, Annamalai University
LAST DATE:
Last date to receive the filled-in application: 1st August, 2011. Upon receipt of the completed application form, the confirmation of registration will be intimated to the participants via email.
For more visit: http://annamalaiuniversity.ac.in/nat_conf_botony2011.htm
HOST DEPARTMENT
The Department of Botany has an Outstanding Record ofpromoting Excellent Research, right from its inception. It has been recognized as DST-FIST & UGC-SAP Sponsored Department. The Department offers 2 year M.Sc. Botany and 5 year integrated M.Sc. Herbal Science Programme. In addition, M. Phil. and Ph.D. programs are offered. The Department has produced 161 Ph.Ds in a variety of botanical disciplines such as Biodiversity and its Conservation, Genetics, Cell and Molecular Biology, Microorganisms and Plant Pathology, Pollution and Environmental impacts on Ecosystem, Plant Physiology and Biotechnology, Plant Taxonomy and Plant Genomics. The Department has organized workshops, seminars and refresher courses through which a huge number of participants from various universities and institutes took part and have placed benefits.
WORKSHOP BACKGROUND
The Polymerase Chain Reaction (PCR) is a technique that permits geometric and enzymatic amplification of specific sequences of DNA. The PCR is now widely used in research laboratories and doctor's offices, relies on the ability of DNA-coping enzymes to remain stable at high temperatures. No problem for Thermus aquaticus, the sultry bacterium from yelllowstone that now helps scientist to produce million of copies of a single DNA segment in matter of hours. In nature, most organisms copy their DNA in the same way. The PCR mimics this process, only in test tubes. When any cell divides, enzymes called polymerase made a copy of the entire DNA in each chromosome. Besides, based on the applications, PCR has been modified in a number of ways to suit specific application situation, several molecular markers (RFLP, AFLP, ISSR, SSR, SNP and RAPD) also available for analyzing genetic variations at molecular level. Among these, RAPD is an inexpensive and a rapid method not requiring much information regarding the genome and has been widely used to document the genetic diversity in plants and animals. Marker-based genome analyses have become increasingly important for various fields of plant science research. In this background, the present workshop will help to provide researchers a hands-on training in molecular marker techniques and to highlight the importance of marker assisted techniques in the plant biotechnology research.
PROGRAMME SCHEDULE
Morning Session : 9.00 am to 1.00 pm;
Lunch : 1.00 pm to 2.00 pm
Afternoon Session : 2.00 pm to 6.30 pm
Tea break in the middle of each session (15min.)
WORKSHOP PROGRAMME
Besides hands-on training, participants will be encouraged to carry out the techniques themselves. The workshop will be conducted for 30 participants only. This workshop of RAPD-PCR technique will cover guest lectures and hands-on experience on various aspects of biotechnology as follows
Laboratory Safety
Tools- Knowledge-information regarding chemicals and other basic instruments required
Major instruments – PCR-thermal cycler, Electrophoresis, Gel documentation.
Chemicals preparation for
DNA isolation and purification.
DNA quantification
Agarose-gel Electrophoresis
PCR reactions
Techniques to be handled
DNA extraction: Isolation and purification of genomic DNA (plant sample).
DNA quantification in spectrophotometer : DNA qualitative analysis in agarose-gel electrophoresis
Amplification in PCR thermal cycler Initial denaturation, Denaturation, Annealing and Extension.
Agarose-gel Electrophoresis, Gel-documentation, Data scoring,Data analysis in software
Results and interpretation
REGISTRATION
The participants are requested to fill-in the Registration form and mail it to the Organizing Secretary along with a demand draft for Rs. 2000/- from Indian Bank / ICICI Bank / SBI in favour of "The Registrar, Annamalai University " payable at Annamalainagar or Chidambaram. Only 30 participants can be accommodated. The first registering persons would be preferred.
VENUE:
Department of Botany, Annamalai University, Annamalainagar-608 002, Tamilnadu.
ACCOMMODATION:
The external participants can be given accommodation for 3days in the university guest house/hostel on request.
For correspondence
Dr. R. SELVARAJ
Organizing Secretary
Professor
Department of Botany
Annamalai University
Annamalainagar – 608 002
Tamilnadu
Tel: Off : (04144)-238282 Ext.354 Fax 238080
Res: (04144)-239014
Email: selvarajphd14@yahoo.co.in
Mobile: 94429 09910
Patron : Dr. M. Ramanathan,Vice-Chancellor, Annamalai University
Vice Patron : Dr. M. Rathinasabapathi Registrar, Annamalai University
President : Dr. A.N. Kannappan Dean, Faculty of Science,
Annamalai University
Chairman : Dr. R. Panneerselvam Prof. & Head, Dept. of Botany,
Organizing Secretary : Dr. R. Selvaraj,Professor, Dept. of Botany,Annamalai University
Joint Secretary : Dr. AL. A. Chidambaram Associate Professor Dept. of Botany, Annamalai University
LAST DATE:
Last date to receive the filled-in application: 1st August, 2011. Upon receipt of the completed application form, the confirmation of registration will be intimated to the participants via email.
For more visit: http://annamalaiuniversity.ac.in/nat_conf_botony2011.htm
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